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Overview
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Assay Validation
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Biomarker-based Trials
Lessons
Terminology and Overview
Clinical Uses of Biomarkers
Learning Objectives:
- Understand the different ways biomarkers are used in oncology clinical practice, including for diagnosis, prognosis, and therapy selection.
- Understand that biomarkers can be based on single measurements or summaries of multiple measurements.
The Use of Biomarkers in Clinical Trials
Learning Objectives:
- Understand the different ways biomarkers are used in clinical trials, including determining eligibility, stratification factors, PK/PD outcomes, and correlative aims.
- Understand the differences between integral, integrated, and exploratory.
- Understand how the level of evidence and background information about the assay depends on its use.
Assay terminology
Learning Objectives:
- Understand the different types of assay inputs, e.g. type and quantity of specimens, reagents, and SOP.
- Understand what an SOP is and why it is important.
- Understand the different types of assay outputs and how they are translated into clinically useful biomarkers.
Lesson Template
Learning Objectives:
- This is the first objective.
- Sentence number two.
- Sentence number 3.
Statistical Issues in Analytical Performance Assessment
Identifying and Describing Different Sources of Variation
Learning Objectives:
- Understand the differences between pre-analytical, analytical, and biological variation.
- Understand statistical properties of the variance, and its decomposition into variance components.
- Understand how to identify what components are estimable in a given study.
Describing and comparing measures of location
Learning Objectives:
- Understand what the term accuracy may mean with a continuous or dichotomous biomarker.
- Understand the statistical properties of bias when there is a reference value.
Statistical issues in design and reporting analytical validation studies
Learning Objectives:
- Understand when to report the coefficient of variation and how to interpret it.
- Sample size planning for assay validation, including negative and positive controls.
- Understand how to describe a study when reporting statistics, including what replicates were performed and how.
Statistical Issues in Biomarker-Based Clinical Trials
Enrichment Designs
Learning Objectives:
- Understand the levels of evidence needed to justify an enrichment design.
- Understand the limitations of the end results from such a design.
- Other pitfalls, including the impact of false positive/negative rates, and partial enrichment.
Integrated Correlative Aims
Learning Objectives:
- Understand the planning process for assessing prognostic biomarkers.
- Understand the planning process for assessing predictive biomarkers, including when the treatment is efficacious overall and not.
- Understand the signature development process.
- Understand the expectations regarding the analysis and statistical plans.
Other
Learning Objectives:
- Stratification factors, and when imbalance is a concern.
- PK/PD outcomes.
- Exploratory correlative aims.